The required volume can be reduced by thickening two or four sides of the bucket inside the walls. Buckets with flat bottom and round bottom are available with AirekaCells. For most flow cells and fluorescence cells, the backgrounds are flat. The Z dimension is usually used with small volume buckets or flow cells when there is a window opening with a limited area. The type 5UV10 is the ultimate balance for someone who is looking for a high-quality UV quartz tamper. The price of the Type 5UV10 is only $54, making it a great purchase for labs on a budget.
However, if you cannot find the buckets you want, please contact us for customization. The position of the light beam passing through the container is determined by the dimension Z. When working with sub-micro volume samples, this is a crucial criterion. Buckets are small rectangular containers made of glass or quartz.
They are usually designed with a path length of 10 mm for the light beam through the solvents, but the length of the pad can be extended from 0.2 or 0.5 mm to 100 mm. The sample cell contains a diluted solution of the solvents spectrophotometer cuvettes to be tested. The main problem is that we cannot define the bucket in this way because of the wide range of materials used to manufacture them. As a result, it’s easy to be perplexed about which option to choose.
Most applications will fall into this range and many do not need the additional UV points obtained with the other materials. As we all know, solutions with a higher concentration should be diluted or use a bucket with a shorter path length to simulate dilution. Known for the Beer-Lambert law, a light bucket range of 1 mm will allow the dsDNA concentration to be up to 1,000 μg/mL. A pair of matching cuvettes describes two buckets of the same material that have the same optical path length.
For UV experiments, you definitely need a UV quartz bucket, and we recommend that you don’t try to cut corners here, because by buying a cheap UV cell, your data won’t be up to par. If you’re on a tight budget, you’ll want an optical glass bucket. This baking material is ideal for working in the VIS range and has a decent transmission range of 340-2,500 nm.
However, glass, plastic and quartz veins are suitable for measuring absorption in the visible area. Budget is an important consideration when selecting laboratory equipment or solutions. Plastic is the most cost-effective bucket of the available options. The optical glass bucket is next, followed by the quartz bucket. So if you’re on a budget, plastic is a great option to consider. Various transparent materials, such as optical glass, quartz or transparent plastic, are widely used to make buckets.